药物RP－HPLC保留值的标准化方法──保留指数系统研究

Standardization for Retention Data of Drugs in RP-HPLC──A Study of Retention Index System

以烷烃甲基酮类作为标准参照物，建立了反相高效液相色谱（ＲＰ－ＨＰＬＣ）的保留指数系统，并探讨了流动相、有机修饰剂浓度对保留指数的影响。这种ＲＰ－ＨＰＬＣ保留数据标准化方法可以减小ＲＰ－ＨＰＬＣ保留值的变异性。药物保留指数随色谱条件变化改变较小，可在实验室间进行比较。该系列参照物不仅可用于紫外检测系统，还可用于示差折光检测系统，本文所建立的ＲＰ－ＨＰＬＣ保留指数系统已在ＯＤＳ和苯基柱上测定了７０多种药物及其代谢物。

A retention index system based on alkan-2-ones was introduced for RP-HPLC.The effects of pH and methanol concentration in the mobile phase on RI were examined.RI of seventy drugs and rnetabolites was determined using ODS-and phenyl-column with UV and RID detectors.The correlation between the lgk' and carbon number was linear from the second homologue. It has been proved that the RI decreases the variability of retention data and is suitable for the identification of drugs and the comparison of retention data obtained from different laboratories,instruments,columns,RI was used for studying QSAR and QSRR of drugs and metabolites because there was a significant correlation between RI and lgP or π.