Skp2、p27^(kip1)在大肠癌中的表达及其临床意义

Expression of Skp2 and p27^(kip1)in colorectal carcinoma and their clinical significance

目的研究Skp2、p27kip1在大肠癌组织中的表达,探讨它们之间的关系及其临床意义。方法采用免疫组化方法检测Skp2、p27kip1蛋白在83例大肠癌、18例大肠腺瘤和20例大肠正常黏膜中的表达。结果大肠癌组织中Skp2蛋白阳性率(28.65±12.60)%,显著高于大肠腺瘤组织(17.28±10.66)%(P<0.01)和大肠正常黏膜组织(6.60±5.54)%(P<0.01)。Skp2蛋白表达与细胞分化程度、肿瘤分期及淋巴结转移呈正相关(P<0.01)。大肠癌组织中p27kip1蛋白阳性率(24.61±11.27)%,显著低于大肠腺瘤组织(49.94±13.22)%(P<0.01)和大肠正常黏膜组织(65.40±15.74)%(P<0.01)。p27kip1蛋白表达与细胞分化程度、肿瘤分期及淋巴结转移呈负相关(P<0.01)。大肠癌组织中Skp2与p27kip1表达呈负相关(r=-0.430,P<0.01)。结论大肠癌中Skp2蛋白的过度表达与p27kip1蛋白降解有关,提示Skp2蛋白可能在大肠癌的发生发展中起重要作用。

Objective To explore the expressions of Skp2 and p27^kip1 in colorectal carcinoma ,and investigate their relationship and clinical significance. Methods The expression of Skp2 and p27^kip2 was detected in 83 colorectal cancers, 18 colorectal adenomas and 20 normal colorectal tissues by immunohistochemical staining. Results The expression of Skp2 protein in colorectal cancer[ （28.65 ± 12.60） % ] was significantly higher than that in adenomas[ （ 17.28 ± 10.66 ） % ] （ P 〈 0.01 ） and normal tissues [ （6.60 ± 5.54 ） % ] （ P 〈 0.01 ）, which had a positive correlation with cell differentiation, Duke＇s stages and lymph node metastasis （ P 〈 0.01 ）. However, the expression of p27^kip1 in colorectal cancer [ （24.61 ± 11.27） % ] was significantly lower than that in adenomas[ （49.94 ± 13.22） % ] （ P 〈 0.01） and normal tissues[ （65.40 ± 15.74） % ] （ P 〈 0.01 ）, which had a significantly negative correlation with cell differentiation,Duke＇s stages and lymph node metastasis（ P 〈 0.01 ）. There was a negative correlation between Skp2 and p27^kip1 in colorectal cancers （ r = - 0. 430, P 〈 0.01 ）. Conclusion Overexpression of Skp2 protein may lead to the decreased p27^kip1 level in human colorectal cancer, indicating its involvement in the carcinogenesis and development of human colorectal cancer.