VEGF和PCNA在前列腺癌组织中表达及临床意义

Expression and clinical significance of vascular endothelial growth factor and proliferating cell nuclear antigen in human prostatic carcinoma

目的探讨血管内皮细胞生长因子和增殖细胞核抗原(PCNA)在同一前列腺癌(Pca)组织中的表达,及与肿瘤血管形成和肿瘤细胞增殖的关系。方法应用免疫组织化学LDP法检测40例Pca,20例良性前列腺增生症(BPE)组织中VEGF及PCNA的表达情况。结果Pca组织中VEGF和PCNA的表达均明显高于BPE;VEGF和PCNA在Pca中的表达水平与其病理分级和临床分期均有关;Pca的VEGF表达水平和PCNA的表达也有关(P<0.01);在VEGF阳性表达病例中PCNA的表达随VEGF表达强度的升高而升高(P<0.05)。结论VEGF和PCNA与Pca组织学分级、恶性程度和预后密切相关,是检测Pca的较好分子标志物。

Objective To study the expression of vascular endothelial growth factor（VEGF） and proliferating cell nuclear antigen（PCNA） in human prostatic carcinoma tissue and their relationship with tumorous angiogenesis and tumor cell proliferation. Methods The expression of VEGF and PCNA in 40 cases of prostatic carcinoma and 20 cases of benign prostatic hyperplasia（BPE） was detected by the immunohistochemical LDP method . Results The expression levels of VEGF and PCNA in prostatic carcinoma were much higher than those in BPE （P〈0.01 ） , which were correlated with the pathological degree and clinical stage of Pca. VEGF level was related with PCNA level as well （ P〈0.01 ）. Besides, the expression of PCNA had positive correlation with VEGF expression in VEGF-positive cases （ P〈0.05 ）. Conclusion This study indicates that VEGF and PCNA are closely related with the histopathological grading, the tumor differentiation and prognosis of Pca. Therefore VEGF and PCNA are effective molecular markers of prostatic carcinoma.