黑根霉对甾体的C_(11)α羟基化反应

STUDIES ON THE REACTION OF STEROID C_(11)α-HYDROXYLATION BY RHIZOPUS NIGRICAN

从黑根霉（Rhizopus nigricans）对16α，17α-环氧黄体酮的C11α-羟基化反应发酵过程曲线开始，考察了碳源、pH对黑根霉的甾体羟基化转化过程的影响．100L发酵罐实验结果表明，采用复合碳源C（葡萄糖：糊精为1：1），转化率比对照提高2％．100L发酵罐实验结果表明，氧化期（从投料开始计）调pH至4．0～5.0，转化率提高3％．

The effects of carbon source and pH on C11α-hydroxylation of 16α, 17α-poxy-4-pregnene-3,20 - dione were studied basing on the figure of steroid C11-αhydroxylation by Rhizopus nigricans. The 100 L bioreactor conversion rate was increased by 2% when using complex carbon source C（glucose：dextrin = 1：1 ）. By adjusting pH to 4.0- 5.0 in oxidation phase the 100 L bioreactor conversion rate was increased by 3%.