1,2,4-三氯苯对小鼠心脏、肝脏和肾脏抗氧化系统的影响

Effects of 1,2,4-trichlorobenzene on anti-oxidation system of heart,liver and kidney in mice

目的探讨1,2,4-三氯苯(1,2,4-TCB)对小鼠心脏、肝脏和肾脏抗氧化系统的影响。方法经皮下注射染毒,分为1 382.2、2 764.4、4 146.6 mg/kg低、中、高3个剂量,测定心、肝、肾脏中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、总巯基(T-SH)、非蛋白巯基(NP-SH)、蛋白巯基(P-SH)及脂质过氧化物(LPO)的含量。结果心脏检测示低剂量组NP-SH含量显著高于对照组;肝脏检测示中、高剂量组LPO含量显著增高,GSH-Px活力和NP-SH含量显著低于对照组,高剂量组SOD活力增高,与对照组相比差异有显著性;肾脏检测示3个剂量组GSH-Px活力有所降低,与对照组相比差异有显著性,低剂量组NP-SH含量显著低于对照组。结论1,2,4-TCB可使小鼠心、肝和肾脏脂质过氧化作用增强,并相应地引起抗氧化酶活力和抗氧化物质含量的变化。

Objective To explore the effects of 1,2,4-TCB on anti-oxidation system of heart, liver and kidney in mice. Method The mice were divided into three experimental groups and given 1 382.2 mg,/kg, 2 764.4 mg/kg and 4 146.6 mg/kg of 1, 2, 4-TCB respectively by subcutaneous injection, then measure the activities of SOD, GSH-Px and the levels of T-SH, NP-SH, P-SH and LPO in heart, liver and kidney. Result The heart measurement showed that, the NP-SH level in low dosage group was higher than that of control group; the liver measurement showed that LPO level in high and middle dosage groups were obviously increased, the GSH-Px activity and the NP-SH level were decresed, the SOD activity in high dosage group was risen compared with control group; while the kidney measurement showed that, the GSH-Px activity in all the three experiment groups were decreased and there was some lower of the NP-SH level seen only in low dosage group. Conclusion 1, 2,4 -TCB could enhanced the lipid pemxidation in heart, liver and kidney of mice and thereby induced the change of the antioxidase activity and anti-oxidant content.