反相离子对色谱法测定硫氰酸根

Reversed-phase Ion-pair Chromatographic Determination of Thiocyanate Anion

本文采用柱前衍生形成［HgEDTA（SCN）3-的方法，实现了对SCN-的反相离子对色谱分离和紫外检测。在ZorbaxODS柱上，用甲醇和水作流动相（甲醇：水为20：80），内含对离子试剂TBA＋4．0×10－3mol／L，EDTA8．0×10－3mol／L，NaNO35．0×10－3mol／L和缓冲溶液KH2PO4－Na2HPO4（PH5．9），检测波长240nm。得到线性关系良好的工作曲线，最小检出限为33．3ng。方法简便，选择性好。

This paper describes a reversed-phase ion-pair chromatographic separation and UV detection of SCN with pre-colurnn derivatization to form [HgEDTA (SCN )]3- complex.The chromatographic processes were performed on a Zorbax oDS column with a mobile phase of methanol-water(20: 80) containing 4. 0 mmol/L TBA+, 8. 0mmol/L EDTA, 5. 0mmol/L NaNO3 and KH2PO4-Na2HPO4 buffcr solution (pH 5. 9), and the eluent was monitored by a UV detector operating at 240nm. The calibration curve is rectilinear and the detection limit is 33. 3 ng. The method is convenient and selective.