新试剂5－氟偶氮胂Ⅰ荧光法测定铝的研究

A New Azo Reagent 5-Fluoroarsenazo-Ⅰand Its Application in the Fluorimetric Determination of Aluminum

本文报道了新试剂５－氟偶氮胂Ⅰ（５－ＦＡｓＡ－Ⅰ）的合成方法，研究了荧光光度法测定铝的反应条件。Ａｌ（Ⅲ）与试剂在ｐＨ５．６０ＨＡｃ－ＮａＡｃ缓冲介质中形成１＋１红色配合物并呈现荧光，λｅｘ／λｅｍ＝３４８．６／３９１．４ｎｍ，线性范围为０～０．２μｇ／ｍＬ，检测限为３．０ｎｇ／ｍＬ。用于分析茶叶样品中的铝，结果满意。

A new reagent 5-Fluoroarsenazo-I has been synthesized,and applied to the fluorimetric determination of aluminum.It is based on the formation of a fluorescent complex of this reagent with aluminum.The red complex formed is of 1:1 type with λex/λem=348. 6/391.4(nm) at PH5. 60 in HAc-NaAc buffer. A linear relationship was obtained over the range of 0～0. 2μg/mL for Al(Ⅲ), while the detection limit is 3.0 ng/mL. The method has been satisfactorily applied to the determination of Al( Ⅲ) in tea samples.