虎杖药材HPLC指纹图谱研究

HPLC fingerprint of Rhizoma Polygoni Cuspidati

目的:建立虎杖药材HPLC指纹图谱测定方法。方法:采用Diamonsil C18柱,流动相为乙腈-0.1%磷酸水溶液线性梯度洗脱,检测波长为230 nm。采用中南大学出版的指纹图谱相似度比较软件进行比较。结果:运用梯度洗脱能很好分离虎杖的各类成分,通过软件的比较,虎杖药材的指纹图谱相似度均大于0.80。结论:本研究所建立的方法可作为虎杖药材质量标准制定参考依据。

Objective： To establish the HPLC fmgerprint of Rhizoma Polygoni Cuspidati （ Polygonum cuspidatum）. Method： The HPLC separation was carded with Diamonsil C18 column and eluted with a gradient from methanol and 0.1% phosphoric acid, the detection wavelength was at 230 nm and recording 70 min. The similarity of chromatograms was compared by mean of the software from Zhongnan University. Result： The constituents of P. cuspidatum were well separated by HPLC,and the similarity was above 0.80. Conclusion： The method can be used for the study of fingerprints of Rhizoma Polygoni Cuspidati.