过敏症豚鼠模型的建立及海虾主要过敏原组分的纯化与鉴定

Establishment of the guinea pig model and the analysis of the main allergen of sea shrimp

旨在建立海虾过敏症豚鼠模型,分析主要过敏原蛋白质组分并将其应用ELISA来提高体外检测的准确性。以海虾蛋白浸液为致敏原,氢氧化铝为佐剂免疫豚鼠,建立豚鼠过敏模型,DEAE阴离子交换纯化主要的过敏原成分,间接ELISA法检测sIgE和IgG。实验结果:模型组血清sIgE和IgG效价分别为18∶0和12∶0480;DEAE阴离子交换层析成功纯化出了海虾中的主要过敏原成分,应用于ELISA检测sIgE效价为13∶20;主要过敏原蛋白检测sIgE效价是蛋白浸液用于检测的4倍。结果表明,海虾过敏症豚鼠模型建立成功,并且纯化的海虾过敏原组分应用于ELISA检测能提高sIgE的检测水平,对海虾过敏症的体外诊断和治疗有一定的意义。

The objective is to establish the guinea pig allergic model, purify the main allergen of sea shrimp, to make sure the main allergen of sea shrimp can be used to improve the sensitivity of allergy tests. The guinea pigs were immunized by injecting with the extracts of shrimp proteins and the adjuvant of aluminum hydroxide. The main allergen of sea shrimp was purified by anion exchange chromatography, and was used to test the titer of slgE and IgG. The slgE and IgG level of experimental groups are higher than that in control, the titer were 1：80 and 1：20480. The purified main allergen was used for the test of the sIgE titer, and the titer of slgE was 1：320, which is four times of the crude extracts of sea shrimp. The animal model is established successfully. The purified main allergen could greatly improve the test of sIgE titer, which suggest that the purified allergen can be used in hypersensitivity diagnosis and therapy of sea shrimp.