酶联免疫吸附法检测黄精皂苷

Analysis of Saponins of Rhizoma polygonati by ELISA Method

利用效价为1：12800（方阵滴定法测得）的兔抗薯蓣皂苷多克隆抗体，建立黄精皂苷酶联免疫吸附（ELISA）检测方法。抗体的最佳稀释度为1：3000，包被抗原的最佳稀释度为1：10000；回归方程I=32.021 logC＋189．02，相关系数R^2=0．9974，最低检测下限I10为2．468×10^-3μg/mL。回收率为80．33％～103.88％，与人参皂苷和强心苷的交叉反应率分别为3．24％和7．48％。测得黄精总皂苷质量分数为0．1366％。

ELISA method to determine the saponins of Rhizoma Polygonati was established by the rabbit with multi-clone antibody having 1 ： 12 800. The optimum diluted ratio of antibody and coated antigen were 1：3 000 and 1 ： 10 000, respectively. The equation was I = 32.021 logC ＋ 189.02. The correlation coefficient was 0.9974 and the lowest limit was 2.468× 10^-3μg/mL. The recovery rate of samples was between 80.33 % and 103.88 %. The rate of intercrossing reaction of saponins of Rh/zoma polygonati with ginseng saponins and cardiac saponins were 3.24% and 7.48%, respectively. The total content of saponins of Rhizoma polygonati was 0. 136 6%.