养殖大黄鱼细菌菌群分离鉴定与分析

Analysis, Isolation and Identification of Bacterial Flora on Cultured Pseudosciaena crocea

对新鲜大黄鱼感官、化学、微生物品质和细菌菌群组成进行定性和定量分析。结果表明,细菌总数为5．51±0．25log10cfu／g,挥发性盐基氮为7．84±2．25mg／100g。分离获得279株细菌,84．2％是革兰氏阴性菌,少量革兰氏阳性菌被检出(6．1％)。优势菌群是肠杆菌科(14．7％)、气单胞菌属(12．5％)、不动杆菌属(11．5％)、摩氏杆菌属(11．1％),并出现了一定比例的假单胞菌属、嗜麦芽窄食单胞菌和其他细菌。肠杆菌科出现较多,表明大黄鱼细菌污染主要来自养殖海区,受非原有菌污染较重,应引起重视。

Sensory, chemical, microbiological quality and bacterial flora on fresh Pseudosciaena crocea were qualitatively and quantitatively investigated. The results showed that total viable counts and total volatile basic nitrogen reached 5.51 ±0. 25Log10cfu/g, 7.84 ±2.25mg/100g respectively. 279 strains of bacteria were isolated and 84.2% of them were gram negative bacteria, a small percentages of gram positive bacteria were detected （6. 1% ） . The predominant bacterial floras were composed of Enterobacteriaceae （ 14. 7% ）, Aeromonas spp. （12.5%） , Acinetobacter spp. （ 11. 5% ） and Moraxella spp. （ 11.1% ） . A few percent of Pseudomonas spp. , Stenotrophomonas maltophilia and other bacteria were also found. The high number of Enterobacteriaceae showed that bacterial contamination on Pseudosciaena erocea was mainly from cultural water field and polluted seriously by non-borne bacteria, and it should be paid attention to.