过氧化物酶体增殖物激活受体-γ在人侵袭性垂体腺瘤中的分布与表达

Distribution and expression of peroxisome proliferator activated receptor-γ in human invasive pituitary adenomas

目的研究过氧化物酶体增殖物激活受体-γ(PPAR-γ)在人侵袭性垂体腺瘤组织中的分布情况和表达水平。方法30例垂体腺瘤组织依据影像学Knosp分级及术中所见分为侵袭组与非侵袭组。免疫组织化学染色分析PPAR-γ在垂体腺瘤组织中的分布情况,W estern B lot分析PPAR-γ在垂体腺瘤中的表达水平,比较PPAR-γ在侵袭性组与非侵袭组之间、各种内分泌类型腺瘤的侵袭组与非侵袭组之间分布与表达差异。结果侵袭组PPAR-γ表达水平明显高于非侵袭性组(P<0.05),侵袭性泌乳素腺瘤、侵袭性无功能腺瘤中PPAR-γ的表达水平显著高于相应的非侵袭组(P<0.05),而在生长激素腺瘤中,侵袭组与非侵袭组间PPAR-γ表达水平无明显差异(P>0.1)。结论PPAR-γ可能在人垂体腺瘤侵袭性方面发挥重要作用,并有可能成为侵袭性垂体腺瘤治疗的新靶点。

Objective To evaluate the distribution and expression of peroxisome proliferator activated receptor-γ （PPAR-γ） in human invasive pituitary adenomas. Methods Thirty consecutive surgically resected pituitary adenomas were divided into invasive group and non-invasive group according to the Knosp grade or exhibitions in operations. Immunohistochemistry was used to confirm the distribution of PPAR-γand the staining positive rates were compared between the two groups. The expression of PPAR-γ was evaluated by Western Blot. Results PPAR-γ immunoreactivity was located in nucleoli of pituitary adenoma cells. The staining positive rate in invasive group was markedly higher than that in non-invasive group （ P 〈 0. 05）. The expression in invasive group was significantly higher than that in non-invasive group （ P 〈 0. 05）. In non-functional and prolactin- secreting pituitary adenomas, the expression of PPAR-γ in the invasive group was more abundant than that in the non-invasive group. There was no significant difference of PPAR-γ expression between growth hormone -secreting invasive and non-invasive adenomas （P 〉0. 1 ）. Conclusion PPAR-γ may play an important role in the invasion of human pituitary adenomas and may be a novel therapeutic target of invasive pituitary adenomas.