中药不同组分对小鼠缺血脑组织蛋白表达谱的影响

Effect of Some Active Chinese Herbal Fraction on Brain Tissue Proteomic Profile of Ischemic Mouse

目的运用蛋白质组学技术分析中药不同组分对小鼠缺血脑组织蛋白表达谱的影响。方法黄芩苷、栀子苷、胆酸、珍珠母分别干预缺血再灌小鼠3h后,断头取脑,提取蛋白,用蛋白质芯片比较分析以上组分对小鼠缺血脑组织蛋白表达谱影响的差异。结果黄芩苷、栀子苷、胆酸、珍珠母可影响5373Da, 5707Da,15103Da 3个靶点蛋白的表达,中药对蛋白表达具有多靶点性和差异性。结论蛋白质芯片技术是探索中药药理机制的有效手段。

Objective To study the effect of .some active Chinese herbal fraction on protein expression of brain tissue in ischemic mouse with proteomic technique. Methods Ischemia-reperfusion mice were treated with baicalin, geniposide, cholic acid and concha margaritifera respectively for 3 hrs, and then their brain tissue were taken to extract the total protein. Protein expression in ischemic mouse brain was analyzed with surface-en- hanced laser desorption/inionation-time of flight-mass spectra （SELDI-TOF-MS） protein-chip. Results The four components tested had effect on 3 target proteins at 5373Da, 5707Da and 15103Da, showing the nature of multi-target and with different action on protein expression. Conclusion Protein-chip is an effective approach for exploring the pharmacological mechanism of Chinese herbal fraction.