心脑舒通胶囊对高脂血症大鼠心肌梗死后心肌细胞凋亡及Bax、Bcl-2蛋白表达的影响

Effect of Xinnao Shutong Capsule on Cardiac Muscle Cell Apoptosis and Protein Expressions of Bcl-2 and Bax in Hyperlipidemia Rats after Myocardial Infarction

目的观察心脑舒通胶囊(主要起效成分为蒺藜总皂苷)对高脂血症大鼠心肌梗死(MI)后心肌细胞凋亡及Bax、Bcl-2蛋白表达的影响,探讨蒺藜皂苷抗高脂大鼠MI后心室重构的作用机制。方法利用高脂血症心肌梗死复合模型,采用TUNEL和免疫组化SP法,观察蒺藜总皂苷对MI后大鼠心肌细胞凋亡、以及Bax、Bcl-2蛋白表达的影响。结果(1)蒺藜总皂苷可减轻MI后心肌细胞损伤和心室重构的程度;(2)与高脂心梗组相比,蒺藜总皂苷大剂量、辛伐他汀均降低MI后心肌细胞凋亡指数,并能下调Bax蛋白表达(P<0．05),各治疗组之间作用比较,差异无显著性(P>0．05)。结论蒺藜总皂苷可通过调控Bax／Bcl-2蛋白表达比值,而抑制心肌细胞凋亡,可能是其抗MI后心室重构的作用机制之一。

Objective To investigate the effect of Xinnao Shutong Capsule, whose main ingredients gross saponins from Tribulus Terrestris L （GSTT） on cardiac muscle cell （CMC） apoptosis and expressions of Bcl-2 and Bax in murine model of hyperlipemia after myocardial infarction （MI）. Methods Compound rat model of MI and hyperlipemia was adopted. TUNEL assay was applied to detect CMC apoptosis after 4 weeks＇ adminis- tration of GSTT or simvastatin, and immunohistochemical SP technique was used to detect the expressions of Bcl-2 and Bax protein. Results GSTT can relieve the damage of CMC and attenuate the ventricular remodeling after MI; high dose of GSTT and simvastatin could decrease CMC apoptosis （P 〈 0.05）, and lower Bax protein expression （P〈0.05）; and there was no significant difference among the effects in all the treated group （P〈0.05）. Conclusion GSTT can reduce CMC apoptosis through regulating protein expressions of Bcl-2 and Bax, which may be one of the mechanisms of its anti-ventricular-remodeling effects after MI.