新荧光试剂3─苯基─5─（4′─硝基─2′─羧基苯偶氮）─2─硫代─4─噻唑啉酮的合成及其应用研究

Synthesis or a new fluorescent reagent 3-phenyl-5-(4'-nitro-2'-carboxylphenylazo)-2-thioxo-4-thiazolinone and its application

报道了新荧光试剂３－苯基－５－（４′－硝基－２′－羧基苯偶氮）－２－硫代－４－噻唑啉酮（３Ｐ－４ＮＲＡＣＰ）的合成，通过元素分析、红外光谱、核磁共振谱及质谱确证了其结构。在ｐＨ５．６时，它与铜（Ⅱ）形成稳定的荧光螫合物，在γｅｘ／λｅｍ＝３０５ｎｍ／４０５ｎｍ处产生强荧光，其荧光强度与铜（Ⅱ）的浓度在１．５７×１０－１０～１．８９×１０－８ｍｏｌ／２５ｍＬ范围内呈线性关系，灵敏度达１．５７×１０－１０ｍｏｌ／ｇ的铜（Ⅱ）。用此方法测定了杂交米、糯米及香米中的痕量铜（Ⅱ），结果满意。

The paper reports the synthesis of a new fluorescent reagent 3-phenyl-5- (4′-nitro-2′-carboxylphenylazo)-2-thioxo-4-thiazolinone(3P-4NRACP).Its composition has been confirmed by elemental analysis,IR,HNMR and MS.At PH5.6,3P-4NRACP can form a fluorescent chelate with Cu(ⅠⅠ).when λex/λem =305um/405nm,the fluorescence is very intense.The fluorescent intensity has a linear relationship with the concentration of Cu2+ in the range of 1.57×10-10～1.89×10-8mol/25mL.The sensitivity is 1.57×10-10 mol/g.Using the method,trace copper has been detected in several kinds of rice with good results.