荧光法研究手性金属配合物与DNA的作用机理

Studies on the Mechanism of the Chiral Metal Complexes with DNA by Fluorimetry Method

以溴化乙锭为荧光探针，研究手性金属配合物［Ｎｉ（ｐｈｅｎ）３］（２＋）和［Ｆｅ（ｐｈｅｎ）３］（２－）与ＤＮＡ的反应机理，结果表明，配合物与ＤＮＡ作用存在插入和静电结合两种模式，即部分菲咯啉配体插入ＤＮＡ双螺旋碱基对中，同时带正电荷的配合物与ＤＮＡ的磷酸基团发生静电结合。

The fluorescence characteristics of EB which is sensitive to DNA was used as a probe to study the interaction of [Ni(phen)3]2+,[Fe(phen)3]2+ with DNA.The results show that the complexes bind to DNA by two models, one is the partial insertion of one of the ligands between the base pairs on the DNA duplex strand, the other is electrostatic binding by which the complexes can interact electrostatically with the phosphate backbone of DNA.