慢性乙型肝炎患者E-选择素A561C基因多态性的研究

The study of A561C polymorpnisms of E-selectin in the chronic hepatitis B

为明确E-选择素(E-selectin)A561C基因多态性与慢性乙型肝炎(CHB)的关系,采用PCR-RFLP技术对91名慢性乙型肝炎患者进行检测。结果显示E-selectin A561C基因存在AA、AC和CC三型,且与慢性乙型肝炎相关(P<0.05),AC+CC基因型的患病风险是AA型的2.89倍,其血清sE-selectin水平显著高于AA型(P<0.01),但肝功能指标差异不显著(P>0.05)。提示E-selectin A561C多态性与慢性乙型肝炎的感染有关,C等位基因可能是慢性乙型肝炎发病的易感因素之一。该位点多态性可影响血清E-selectin水平,但对肝功能损伤不起直接的作用。

To study the correlation of E- selectin A561 C polymorphisms and chronic hepatitis B, E- selectin A561 C polymorphisms were detected by PCR - RFLP in 91 （CHB） patients. Three genotypes （ AA,AC and CC） were found which were an association with chronic hepatitis B （P 〈 0. 05）. The relative risk of suffering from chronic hepatitis B of genotype AC ＋ CC was 2. 89 times of the AA genptypes. Meanwhile serum sE - selectin level was sighificant higher than that of AA caniers markedly （ P 〈 0. 01 ）. However the markers of liver injury didn show significant difference （ P 〉 0. 05 ）. Therefore there is an association between E - selectin A561C polymorphisms and chronic hepatitis B infection. Allele C may be one of predisposing factors chronic hepatitis B, its polymorphism has an effect on serum E - selectin level, but not on liver injury directly.