SMMC-7721肝癌细胞膜上β_2糖蛋白I可能的受体

A possible receptor for beta-2-glycoprotein I on the membrane of hepatoma cell line SMMC-7721

研究β2糖蛋白I(β2GPI)与肝细胞相互作用的过程,以进一步探讨β2GPI在乙型肝炎病毒感染肝细胞过程中所发挥的作用。采用L igand b lot技术,从SMMC-7721、HL-60及SGC-7901三个细胞株中,筛选出具有与人β2GPI特异结合蛋白成分的细胞。SMMC-7721细胞在40kD处出现一特异染色带,而HL-60及SGC-7901二种细胞则无此反应。在SMMC-7721细胞中存在有与人β2GPI特异结合的蛋白,这种蛋白可能参与了HBV感染肝细胞的过程。

To study the interaction of beta - 2 - glycoprotein Ⅰ （ β2 GPI ） with hepatocye, and further to explore whether β2GPI play an important role in hepatitis B virus （HBV） infection. Using Ligand blot technique, we detected the interaction of β2GPI with the hepatoma cell line SMMC -7721, the gastric carcinoma cell line SGC -7901 and the lymphoma cell line HL- 60. A specific 40kD β2GPI band was observed in the case of SMMC -7721. No such band was observed in HL -60 and SGC -7901. There is the specific β2GPI binding protein in SMMC -7721. This protein maybe participates in HBV infection.