摘要
目的 建立RP—HPLC测定腰息痛肢囊中芍药苷的含量。方法 采用Kromasil C18柱，以乙腈-0.1％磷馥溶液（15：85）为流动相，检测波长230nm。结果 芍药苷在20～80μg·ml^-1范围内呈良好线性关系，平均加样回收率为98.8％,RSD=1．2％（n=5）。结论 反相高效液相色谱法洲定腰息痛肢囊中芍药苷的含量，方法准确、灵敏、回收率高、专属性好。
OBJECTIVE To establish an RP-HPLC method for determination of paeoniflorin in Yaoxiteng Capsules. METHODS ODS column was used. The mobile phase was acetonitrile-0. 1% H3PO4 solution（15 ： 85）,the detection wavelength was 230nm. RESULTS The linear range of paeoniflorin was within 20-80μg· ml^-1. The average recovery was 98. 8 %, RSD = 1.2 （ n = 5）. CONCLUSION The method was accurate and sensitive with a good recovery.
出处
《齐鲁药事》
2006年第6期335-337,共3页
qilu pharmaceutical affairs
