维多利亚蓝B分光光度法测定硫酸软骨素

Spectrophotometric Determination of Chondroitin Sulfate with Victory Blue B

在pH4．0醋酸-醋酸钠缓冲液中，阳离子染料维多利亚蓝B在614nm处有一吸收峰。它与硫酸软骨素（Chs）反应生成缔合微粒后导致614nm处的吸收峰降低，其吸光度差值与Chs浓度在0．1～5μg·mL^-1范围内呈良好的线性关系。据此建立了一个简便、快速、准确测定硫酸软骨素含量的分光光度新方法。该法已用于合成样及康得灵注射液样品分析，结果满意。

In pH 4. 0 acetic acid-sodium acetate buffer solution, cationic dye victory blue B shows an absorption peak at 614 nm, and the absorption peak decreases after it reacts with chondroitin sulfate to form association particles. The decrease in absorption value is linear with chondroitin sulfate concentration in the range of 0.1-5μg·mL^-1 , and the correlation efficient is 0. 998 6. The method was applied to the determination of chondroitin sulfate in synthesis samples and real samples with rapidity, simplicity and good accuracy.