水稻中一个LRR-RLK基因OsInlk2的克隆与表达分析

Cloning and Expression Analysis of a Leucine-rich Repeat Receptor-like Kinase Gene (OsInlk2) in Oryza sativa

富含亮氨酸重复序列的类受体蛋白激酶(LRR-RLKs)在植物的生长发育过程中起到非常重要的作用,如细胞识别,抵抗病原体的入侵以及自交不亲和反应.现已克隆了一个水稻LRR-RLK基因OsInlk2,并对它进行了序列和表达分析,结果表明OsInlk2由三个部分组成,即胞外LRR结构域,跨膜区域和蛋白激酶区域.种内变异分析表明OsInlk2在籼稻中表达,而在粳稻中却不表达.另外组织表达分析表明OsInlk2在茎和幼穗中的表达量很高,而在叶片和根中的表达量较低.对珍汕97B三叶期幼苗进行盐胁迫和冷胁迫,结果表明随着NaCl浓度的提高,OsInlk2的表达呈先减后增的趋势;4℃低温处理8 h,该基因的表达量比对照降低,但随着处理时间的延长,表达量却急剧上升.

Leucine-rich repeat receptor-like kinases （LRR-RLKs） play key roles in the cell-cell recognition process during development, defense against pathogens, and self incompatibility. The cloning, characterization, intraspecies variation and stress-resistance reaction of putative LRR-RLKs gene （OsInlk2） in Oryza sativa were presented. SMART analysis shows that the deduced amino acid contains extracellular leucine-fich repeats, a single transmembrahe domain, and a cytoplasmic kinase domain. Intraspecies variation analysis reveals that expression of OsInlk2 existed in Indica type rice, while it did not exist in Japonica rice. The tissue-related expression pattern of Oslnlk2 shows that the mRNA level was extensively high in stem and immature seed, while it was much lower in leaf and root. Cold treatment and salt treatment analysis revealed that the expression of Oslnlk2 was affected by low-temperature, treating time and salt concentration.