运用共振拉曼散射光谱法测定皮肤类胡萝卜素

Detection of Skin Carotenoids with Resonance Raman Spectroscopy

[目的]用共振拉曼散射光谱法测试皮肤类胡萝卜素水平,观察机体类胡萝卜素变化情况。[方法]120名成年受试者按照其年龄、性别和试验前皮肤类胡萝卜素指数配对后,随机分为试验组和对照组。试验组人群每天补充β-胡萝卜素12.6mg,共8周。两组人群在试验前、试验第4周末和第8周末,分别运用共振拉曼散射光谱测试受试者皮肤类胡萝卜素,观察皮肤中类胡萝卜素水平变化情况。同时进行膳食调查,了解膳食摄入情况。[结果]试食组人群补充β-胡萝卜素第4周时皮肤类胡萝卜素指数比试验前的基础值上升了24.3%、第8周时比试验前的基础值上升了44.0%,且与对照人群相比差异有统计学意义(P<0.001)。[结论]由于两组人群在试验期间的膳食摄入情况没有差异,实验中试食组人群皮肤类胡萝卜素指数的增高可认为与补充β-胡萝卜素有关。共振拉曼散射光谱作为一种非创伤性的检测方法,可用于反映皮肤类胡萝卜素水平,进而提示机体类胡萝卜素的营养状况。

[ Objective ] To observe the variety of carotenoids level in the body through the detection of skin carotenoids. [ Methods ] 120 adult subjects were paired according to age, gender and the level of skin carotenoids at entry, and divided randomly into treatment and control groups. The treated subjects took 12.6 mg β -caroteneid per day for 8 weeks. At the beginning, 4th and 8th week, the skin carotenoids was detected with resonance raman spectroscopy. Meanwhile, 24h-dietary questionnaires for all participants were carried out. [ Results ] After supplementation, the value of skin carotenoids went up 24.3% at the 4th week and 44.0% at the 8th week over the beginning in the treatment group, and there was a very significant difference comparing with the control group （ P 〈 0.001 ） . The intakes of food and dietary β -carotene were not significantly different between two groups during the study. [ Conclusion ] The increase of skin carotenoids in treatment group may relate to the supplement of β -caroteneid. Raman scattering method, as a non-invasive method, is useful to reflect the level of carotenoids in human body through the measurement of carotenoids in skin