异丙草胺原药致突变性试验研究

Study on Mutagenicity of Propisochlor

目的探讨异丙草胺原药的致突变性,预测其遗传危害和潜在致癌作用的可能性。方法用昆明种小鼠经口灌胃染毒,骨髓微核试验的剂量分别为5000、2500、1250、625mg/kg,睾丸细胞染色体畸变试验的剂量为625.00、312.50、156.25mg/kg,分别取胸骨、睾丸组织,常规制片,镜下观察。鼠伤寒沙门氏菌回复突变(Ames)试验采用平板掺入法,剂量分别为0.5、1.0、2.0、5.0mg/皿。结果小鼠骨髓微核、睾丸细胞染色体畸变试验显示:异丙草胺原药染毒组与阴性对照组比较,差异无显著性(P>0.05);Ames试验显示各菌株的各剂量组的回变菌落数均未超过自发回变菌落数的两倍。结论在本试验条件下,未发现异丙草胺原药的致突变性。

Objective To study the mutagenic effects of propisochlor and predict its potential genetic and carcinogenic effect. Methods The tests on the micronucleus of bone marrow cell and chromosome aberration of testis cell and Ames test were carried out. The Kunming mice were used and gavaged prepisochlor with dose 5000, 2500, 1250 and 625mg/kg for micrenucleus test, and 625, 312.5, 156.25 mg/kg for chromosome aberration test. For Ames test each plate exposed the dose of 0.5, 1.0, 2.0 and 5.0mg. Results The rate of micrenucleus and chromosome aberration in all experimental groups was as low as that in the negative control group. No significant difference was noted. The counts of revertant colonies induced by tested chemical were not as high as two times of spontaneous revertant colonies. Conclusion Propisochlor might have no mutagenicity under present study.