绿帝王组织培养快速繁殖技术

Rapid Micro-propagation Techniques for Philodendron erubescens 'Ludiwang'

选取绿帝王盆栽大苗作为供试母株,对绿帝王组织培养快速繁殖技术进行了研究。结果表明:绿帝王组培外植体最佳取材时间应在冬、春季;不定芽分化增殖阶段最佳培养基为M S十6-BA 3.0m g/L十NAA 0.3 m g/L;最佳生根培养基为1/2M S十NAA 0.5 m g/L十蔗糖20 g/L十活性碳2.0 g/L,和1/2M S十IBA 2.0 m g/L十蔗糖20 g/L十活性碳2.0 g/L;苗高达4 cm,有4片以上叶子,1条~3条以上根系的健壮试管苗,放温室进行3 d^15 d的闭瓶炼苗,7 d^10 d开瓶炼苗,移栽成活率可达95%以上。

Using Philodendron erubescens ‘udiwang＇ seedling-in-pot as test material, its micro-propagation techniques was obtained. The results showed that Winter and Spring are the optimum time for explant ; Differentiation of adventitious bud was in MS medium culture with 6-BA3.0 mg/L ＋NAA0. 3 mg/L ; The best medium for rooting was 1/2MS＋NAA0. 5 mg/L＋sucrose20 g/L＋activated carbon2.0 g/L and 1/2MS＋IBA2. 0mg/L＋sucrose20 g/L＋activated carbon 2.0 g/L;The plantlets, with height of 4 cm, leaves of 4 or above, roots of 3 or 4, was cultured in sealed bottle in greenhouse for 3-15 d, then trained in sealed bottle for 7-10 d, so the survival rate of transplanted plantlets was over 95 %.