三七茎基与须根含量测定方法的研究

STUDY OF DETERMINATION OF LUTOU AND FIBROUS ROOT OF ITS RADIX

目的:建立三七茎基与须根中皂苷的含量测定方法,测定不同药龄和不同月份生育期的广西三七茎基与须根中皂苷的含量。方法:采用RP-HPLC法,ShimpackCLC-ODS柱分离,流动相:乙腈与水梯度洗脱,检测波长203nm,柱温:室温,流速1·0ml/min。结果:广西三七茎基与须根中人参皂苷Rg1、Re、Rb1和三七皂苷R1的含量随药龄及月份的变化而变化;所含的Rg1和Rb1的总量均符合《中国药典》2000年版中三七的质量要求。结论:所建立的高效液相色谱法灵敏度高,专属性强,重现性好,能准确、快速的进行含量测定;广西三七茎基和须根中Rg1、Re、Rb1和R1的含量普遍较高,完全可与三七主根一起入药。

Objective：To establish a new method to determine the contents of notoginsenoside of jinji and fibrous root in its radix notoginseng in different growth periods. Methods： A RP-HPLC method was set up by Shim pack CLC-ODS column and gradient elution with acetonitrile and water. Diode array detector was set at 203 nm and column temperature was room temperature. The flow rate was 1.0 ml/min. Result：The results showed that the contents of notoginsenoside varied with different growth periods and colletion times of the plant. The contents of ginsenoside Rg1 and Rb1 were in accordance with China Pharmacopeia of year 2000 edition. Conclusion：This RP-HPLC method is simple, specific and accurate. Ginsenside Rg1 ,Re and Rb1 and notoginsenoside RL from jinji and fibrous root of radix notoginseng are all relatively rich so that they can be completely used like radix notoginseng.