香花槐组织培养及快速无性繁殖技术研究

The Research of Tissue Culture and Rapid Non-gender Propagetion of Robinia cv.Idaho

以香花槐幼叶,幼茎为材料,进行组培和快速繁殖研究。结果表明:诱导培养基为MS+BA1.0mg.L-1+NAA0.05mg.L-1时有利于愈伤组织的形成;MS+BA0.5mg.L-1+NAA0.1mg.L-1+IBA0.2mg.L-1时增殖倍数为3-4,且生长良好;生根培养基则用1/2MS+NAA0.05mg.L-1+IBA0.2mg.L-1可使生根率达95%以上。

With the leaflet and stemlet of Robinia cv.Idaho as experiment materials, the experiments of tieeue culture and rapid propagation have been done respectively and the result indicates：heal tissue easily formed at the inducing medium level of MS＋BA1.0mg.L^-1＋NAA0.05mg.L^-1,breeding multiple is 3-4 and the plants grow well at the level of MS＋BA0.5mg.L^-1＋ NAA0.1 mg.L^-1＋IBA0.2mg.L^-1,regenerating ratio can reach more than 95% at the root-shooting medium level of 1/2MS＋NAA 0.05mg.^-1＋IBA0.2mg.^-1.