^(125)Ⅰ粒子组织间植入对小鼠移植性肝细胞癌的治疗作用

The experimental study on hepatocellular carcinoma mice treated with ^(124)I seeds interstitial brachytherapy

目的探讨(125)~I 粒子组织间植入对小鼠移植性肝细胞癌的治疗效果方法在70只小鼠背侧皮下建立 H22移植性肝细胞癌模型,选50只移植成功的荷实体瘤小鼠,其中治疗组40只按植入肿瘤的(125)~I 粒子活度不同分为A、B、C、D 4组;另10只作为对照组,于肿瘤内植入无活性的空心粒子。植入(125)~I 粒子后,连续观察30 d小鼠存活情况,测量肿瘤大小,分别对治疗后肿瘤组织进行细胞学分析,观察(125)~I 粒子对肿瘤组织的破坏程度和范围。结果 (125)~I 粒子植入30 d 后,治疗各组(A、B、C、D)和对照组小鼠存活率分别为80%、80%、60%、60%和40%;肿瘤的平均体积分别为(203.8±76.5)、(235.7±88.7)、(518.2±178.4)、(965.5±310.3)和(3242.4±879.8)mm^3,治疗各组与对照组比较差异有显著性(P 均<0.01)。细胞学检测示治疗各组(125)~I 粒子周围肿瘤细胞有不同程度变性、坏死。结论 (125)~I 粒子植入治疗小鼠移植性肝细胞癌效果明显,其疗效与肿瘤的周边剂量有关。

Objective To study the therapeutic effect of interstitial brachytherapy with ^125I seeds in hepatocellular carcinoma mice. Methods Fifty of 70 hepatocellular carcinoma H22 cells bearing mice models were successfully prepared, and 40 of them were divided into four groups. In each treated group, the ^125I seeds were implanted into the tumor with different radioactivity, whereas in the control group （10 mice） the dummy seeds were implanted. The survival rates, the tumor dimensions of mice were observed during 30 d after implantation. Routine pathological study of tumor tissues was studied under light microscope to evaluate the severity and extent of tumor tissues damaged by 12.51 seeds. Results The survival rates of each treated group （A, B, C and D） and the control group were 80%, 80%, 60%, 60% and 40% respectively; the average volumes of tumors in corresponding group were （203.8 ± 76.5 ）, （ 235.7 ± 88.7 ）, （ 518.2 ± 178.4）, （965.5 ± 310.3 ） and （ 3242.4 ± 879.8） mm^3 respectively （ P 〈 0.01 ）. Pathological examination showed various degree coagulative and degenerative necrosis of tumor cells presented at the periphery of active ^125I seeds. Conclusion The interstitial brachytherapy with ^125I seeds can significantly inhibit the growth of hepatocellular carcinoma cells of mice and there are close relationship between the therapeutic effect and peripheral dose of the tumors.