补肾活血法对子宫内膜异位大鼠腹腔液细胞因子变化的影响

Effects of Curative Methods with Tonifying the Kidney and Promoting Blood Circulation by Removing Blood Stasis on Cytokine Production in Intraperitoneal Fluid of Endometriosis Model Rats

目的探讨不同中药制剂对子宫内膜异位症大鼠腹腔液中IFN-r,IL-2,IL-10变化的影响。方法对3组每组6只子宫内膜异位症大鼠分别采用补肾活血法、活血化瘀法、单纯补肾法灌胃4周后测定腹腔液中IFN-r,IL-2,IL-10含量,与采用生理盐水灌胃的对照组进行比较。结果与对照组相比较,补肾活血法治疗后可以显著增加子宫内膜异位症大鼠腹腔液中IFN-r,IL-2的含量(P<0.01),同时亦使IL-10含量显著降低(P<0.01)。而其它治疗组则无IFN-r,IL-2及IL-10的同时变化。结论补肾活血法较活血化瘀、单纯补肾法治疗子宫内膜异位症应具有更好的疗效,因为补肾活血法可以更显著的恢复子宫内膜异位症大鼠腹腔液中Th1/Th2细胞的动态平衡,从而恢复以Th1型细胞为主介导的细胞免疫功能。

Objective To investigate the effects of different herbs on the cytokine variation in intraperitoneal fluid of endometriosis model rats. Methods 24 model rats with endometriosis were separated randomly into 4 groups （ n = 6） ： group A treated with tonifying the kidney and promoting blood circulation, group B with promoting blood circulation by removing blood stasis, group C with simple tonifying the kidney, and group D with normal .saline as control. The levels of IFN - r, IL- 2 and IL- 10 in intraperitoneal fluid were detected. Results The levels of IFN r and IL- 2 increased and IL- 10 decreased significantly in group A compared with the control group （P〈0.01）. There was not significant difference in IFN- r, IL 2 and IK - 10 between group B or C and the control group. Conclusions The curative effect of tonifying the kidney and promoting blood circulation is better than promoting blood eirculation by removing blood stasis or simple tonifying the kidney in the treat ment of endometriosis.