摘要
目的探讨卡介菌多糖核酸(斯奇康)对结核性胸膜炎的免疫调节作用机制。方法用双抗体夹心酶联免疫吸附试验(ELISA)和间接酶标记的链酶卵白素—生物素检测法,观察卡介菌多糖核酸治疗前后结核性胸膜炎患者胸腔积液中可溶性细胞间粘附分子-1(sICAM-1)和外周血淋巴细胞上粘附分子配体CD11a,CD11b阳性表达百分率。结果经卡介菌多糖核酸治疗后,患者胸腔积液中sICAM-1及外周血CD11a,CD11b表达显著降低(P<0.001),且10例患者胸腔积液完全吸收。结论卡介菌多糖核酸对结核性胸膜炎的免疫调节可能通过影响淋巴细胞上粘附分子的表达及细胞间粘附连接实现,以此抑制结核性胸膜炎的细胞免疫反应,减少胸腔积液渗出,控制患者病情。
Objective To investigate the immunological mechanism of BCG-PSN ameliorating tuberculous pleurisy. Methods ELISA and avidin- biotin complex method were used to observe the positive expression percentage of adhere molecule C11a, and CD11b of lymphocyte in tuberculous pleurisy without and after using BCG-PSN, and compare the expression of slCAM-1 in tuberculous pleural fluid before and after using BCG-PSN. Results The levels of slCAM - 1 in tuberculous pleural fluid and the expressive levels of adhere molecule CD11b, and CD11b on the peripheral blood lymphocytes were lower significantly after using BCG- PSN（ P 〈0. 001 ）, and tuberculosis pleural fluid was absorbed absolutely in 10 patients. Conclusion BCG- PSN maybe immunoregulate tuberculous pleurisy by affecting the expression of adhere molecules and conjunctions of intercell, which restrains cell immune and is beneficial to ameliorate the tuberculous state of an illness and reduces pleural effusion.
出处
《中国药业》
CAS
2006年第6期42-43,共2页
China Pharmaceuticals
