摘要
通过三亲本杂交将携带豌豆根瘤菌吸氢基因的质粒ｐＡＬ＿(６１８)转移到台湾毛豆根瘤菌２９２－Ｃ中，经抗性筛选、质粒检测和吸氢活性测定，得到能稳定遗传的结合株２９２－Ｃ２和２９２－Ｃ３。在自生条件下结合株均表现较高的吸氢活性，而受体株２９２－Ｃ不吸氢，在共生条件下结合株表现为高吸氢，而受体株表现为放氢。
The cosmid pAL618 containing the hydrogen uptake genes of Rhizobiumleguminosarum was transferred into Rhizobium Japonicum（Glycine max meer 292-C）bytriparent mating system，Two transconjugants 292-C2 and 292-C3 were selected in Rhizo-bium mininal medium containing Ampicillin（25mg／L）and Tetraeycline（10mg／L）．Theexpression of hydrogenase activities of transconjugants were studied．The transconju-gants 292-C2 and 292-C3 showed high hydrogenase activity both in free-living and symbi-otic state whiie the recipient showed no hydrogen uptake activity in free-living state andhydrogen evolution in symbiotic state．
基金
福建省自然科学基金
