分子杂交技术在人乳头瘤病毒分型检测中的应用

The application of molecular hybridization in typing of human papilloma virus

目的了解人乳头瘤病毒(HPV)型别(低危型:HPV6/11/42/43/44,高危型:HPV16/18/31/33/35/39/45/51/52/53/56/58/59/66/68/73/83/MM4)的感染和分布情况,为HPV感染的诊断和相关疾病的防治提供依据。方法利用核酸扩增和分子杂交技术检测606例临床送检样本23种HPV型别,对检测结果进行统计分析。结果HPV阳性检出率为21.8%,其中单一型别感染率为17.5%,两型混合感染者2.8%,三型混合感染者1.5%。HPV型别分布情况为:HPV16型21.2%、HPV11型18.8%、HPV33型12.1%、HPV58型11.5%、HPV18型9.7%、HPV6型7.9%,其余型别为18.8%。结论本法对HPV进行基因分型,可同时检测23种HPV型别,并检出具体的感染型别,为HPV感染诊断和宫颈癌防治提供重要依据。

Objective To comprehend the status and infection of human papilloma virus （HPV） genotypes and provide evidence for the diagnosis. Methods 606 samples were examined by nucleic acid arnplification （NAT） and molecular hybridization to genotypes of HPV. Then statistical analysis was taken. Results 21.8% of those samples were checked out HPV. Among them, 17.5% were infected by single HPV genotype, 2.8% were infected by two genotypes and 1.5% were infected by three genotypes. The HPV subtypes showed that 21.2% were checked out HPV16, and with 18.8% of HPV 11, 12.1% of HPV 33, 11.5% of HPV 58, 9.7% of HPV 18, 7.9% of HPV 6 and others of other HPV genotypes respectively. Conclusions The methods of nucleic acid amplification and molecular hybridization can check out 23 kinds of HPV genotypes in the same sample. It can provide the important evidence in diagnosing HPV and the prevention and cure for cervical carcinoma.