氨基酸为显色剂比色法测定京尼平的含量

Determination of Genipin by Colorimetry Using Amino Acid

建立了一种京尼平含量的快速测定方法。研究了京尼平与氨基酸的显色反应条件,京尼平(0.0768mg·mL1)与氨基酸溶液(0.2mg·mL1)体积比为1:1,100℃下显色反应10min,检测波长590nm。该方法检测线性范围为0.76～30.73·g·mL1,线性回归方程为Y=0.2409X+0.0036,相关系数为0.9998,最低检测限为0.38μg·mL1,精密度为1.6%。本方法操作简便、快速,且结果准确可靠。

A colofimetry method was established to determinate genipin.The optimal color reaction temperature is at 100℃ for 10 min with the volume ratio of genipin solvent（0.0768 mg·mL^-1） to Arg solvent（0.2 mg·mL^-1） 1：1. The method＇s measurement range is from 0.76 μg·mL^-1 to 30,73 μg·mL^-1 ,the linear correlation is 0.9998,the detection limit is 0.38 μg·mL^-1, RSD is 1.6%. This method has the advantages of wide measurement range, low detection limit, and simple operating process, and can be used for quantitative determination of genipin.