摘要
本研究通过单因素实验,以提取的选择性和提取率为指标,考察了白花蛇舌草中熊果酸的提取过程的几个影响因素;并通过正交实验,对过程参数进行了优化。结果表明,各因素对熊果酸提取效果的影响程度从高到低分别是:提取时间>提取次数>乙醇体积分数>液固比;确定的最适提取条件为:95%乙醇溶液在回流温度下提取两次,每次1.5h,液固比为8:1。
To optimze the condifions for the extraction of ursolic acid from Hedyotis diffusa. Willd, extraction was studied by single factor and orthogonal experiment guided by the recovery of ursolic acid. Results showed that the successive order of factors affecting extraction was extraction time 〉 extract times 〉 alcohol concentration 〉 solid-liquid ration. The optima conditions for extraction of ursolic acid were detemained to be 95% alcohol at 85 ℃ in 1.5h for twice and solid to liquid ratio 1：8.
出处
《天然产物研究与开发》
CAS
CSCD
2006年第3期474-477,共4页
Natural Product Research and Development
基金
2004年浙江省中医药科研基金(2004C036)
关键词
白花蛇舌草
熊果酸
提取
Hedyotis diffusa. Wind
ursolic acid
extraction
