摘要
本研究通过单因素实验和正交实验,采用HPTLC检测方法,以苦瓜甾苷浸提量为考察指标,对影响苦瓜甾苷提取效果的各因素进行了研究。结果表明各因素对苦瓜甾苷提取效果的影响程度从高到低依次为:乙醇浓度>固液比>浸提时间>浸提温度。确定了苦瓜甾苷的最佳提取工艺参数为乙醇浓度80%,浸提温度55℃,浸提时间5h,固液比1:10。
To optimize the condition for extracting charantin from Momordica charantta, extraction process was studied by single factors and orthogonal experiment guided by the content of charantin determined by HPTLC scan. The results showed that the successive order of factors affecting the extraction was ethanol concentration, ratio of solid to liquid, extraction time and temperature. The optimal methods for the extracting charanfin were to extract the material with 80 % ethanol at 55℃ in 5 h with solid to liquid ratio 1 ： 10.
出处
《天然产物研究与开发》
CAS
CSCD
2006年第3期487-490,共4页
Natural Product Research and Development
关键词
苦瓜
HPTLC
胡萝卜苷
苦瓜甾苷
正交设计
提取工艺
Mornedica charantia L.
HPTLC
daucosterol
charantin
orthogonal experiment
extraction process
