摘要
利用实验室已有的17株黑曲霉单宁酶活性菌株为起始菌。经活化分离初筛,液体摇瓶复筛,选出具有高单宁酶活性的No.12菌株;对该菌株进行液体培养,提取单宁酶并固定化;以固定化单宁酶处理滇橄榄汁。结果表明,处理后的滇橄榄汁,固体悬浮物下降90%,说明本工艺具有潜在的工业开发价值。
Aspergilus niger No. 12 with high tannase activity was selected from 17 strains in the laboratory through several steps, including primary screening, two-stage liquid fermentation, biotransformation and TLC analysis. This strain was cultured in liquid media. The tannase was extracted and immobilized. It was used to treat Fructus phytlanthi juice. The results showed that solid substances in the juice were decreased 90% and this technology had the potencial value of the industrial development.
出处
《天然产物研究与开发》
CAS
CSCD
2006年第B06期16-18,共3页
Natural Product Research and Development
基金
中国科学院与攀枝花市院地合作孵化基金
关键词
黑曲霉
单宁酶
固定化
滇橄榄
Aspergilus niget
tannase
immobilization
Fructus phyllanthi.