摘要
研究了阳离子聚丙烯酰胺絮凝剂与硅藻土助滤剂配合施用对α-淀粉酶发酵液的絮凝处理工艺。结果表明:适宜的絮凝条件为pH 7．0-7．5,温度30-35℃,阳离子聚丙烯酰胺用量0．4-0．5 g／L,硅藻土用量20 g／L,此时菌体絮凝率(FR)可达95％以上。α-淀粉酶发酵液经絮凝处理后,过滤速率 (FV)为未处理的9．36倍,滤液澄清率(TR)在98％以上,α-淀粉酶活力回收率达92％以上。
Treatment process of cationic polyacrylamide and diatomaceous earth for the flocculation of the α-amylase fermented broth was studied. The result showed that the flocculation ratio （FR） was more than 95% under the optimum flocculation conditions, where the pH of 7.0-7.5, temperature of 30-35℃, flocculant concentration of 0.4-0.5 g/L and diatomaceous earth concentration of 20 g/L. In comparison with a zero flocculant control, the filtration velocity （FV） and filtrate transparency ratio （TR） of α-amylase broth treated with flocculant were 9.36 fold and more than 98%, respectively, and the recovery ratio of α-amylase activity was more than 92%.
出处
《江苏食品与发酵》
2006年第2期1-4,共4页
Jiangsu Food and Fermentation
关键词
α-淀粉酶发酵液
絮凝剂
絮凝
过滤
α-Amylase fermented broth
Flocculation
Flocculant
Filtration
