慢性应激对大鼠主动脉超微结构和血管细胞粘附分子-1表达的影响

The Affect of Chronic Stress to Ultrastructure and Expression of Vascular Cell Adherence Molecules-1 in Thoracic Aorta in Rats

目的:研究慢性应激对主动脉超微结构和主动脉血管细胞粘附分子-1(VCAM-1)表达的影响。方法:将健康的24只Wistar雄性大鼠随机分成实验组和对照组各12只。适应性饲养1周后,实验组大鼠给予3周应激刺激制作慢性应激动物模型,对照组不给予任何刺激正常饲养。两组大鼠同时处死,用透射电镜观察主动脉组织的超微结构;用免疫组化方法检测主动脉血管内皮细胞VCAM-1的表达情况。结果:①实验组大鼠主动脉的超微结构改变,内皮细胞的线粒体明显肿胀,空泡变。而对照组大鼠的主动脉内皮细胞超微结构无此改变;②实验组大鼠主动脉组织的VCAM-1表达增强(平均灰度值为151.39±1.17),显著高于对照组(其平均灰度值为159.48±3.75,P<0.05)。结论:①慢性应激能造成大鼠主动脉超微结构的改变,内皮细胞线粒体结构退行性变和内皮细胞的炎症性变;②慢性应激能导致大鼠主动脉血管内皮细胞VCAM-1的表达增强。

Objective： Studying the affect of chronic stress to uhrastructure and the expression of vascular cell adherence molecules-1 in thoracic aorta in rats. Methods： 24 male Wistar rats are divided into two groups randomly, including the test group and control group. The number of each group is 12. The test group are compelled to receive a series of stimulation after they are fed 1 week in quiet surrounding, but the control group are not. All of rats are executed at the same time. After have drawn the materials from the thoracic aorta, we observe the uhrastructure by electronic microscope and the expression of vascular cell adherence molecules-1 bv the method imlnunohistochelnistrv in thoracic aorta. Results： ①The uhrastructures in test group are changed, but the control group are normal; the modification is concentrated on the obvious swelling, vacuolicitical transformation in mitochondria, but the control are not. ②The VCAM- 1 is highly expressed in thoracic aorta of the test group, there is not in control group（P〈0.05）. Conclusion： ①The chronic stress can exchange the uhrastructure in thoracic aorta in rats. the core of modification is that there occurrence the degenerative change in mitochondria of endothelial cell and inflammatory, change in endothelial cell ②The chronic stress can lead to the majority expression of VCAM-1 in the endothelial cell membrane.