摘要
目的观察大鼠脑挫伤后神经细胞DNA片段化和DNA含量的时序变化规律。方法建立自由落体打击大鼠脑挫伤动物模型,采用TUNEL和Feulgen′sDNA染色方法,结合图像分析技术进行研究。结果大鼠脑挫伤后随着损伤时间延长DNA片段化程度逐渐增强,而DNA含量逐渐降低。结论采用TUNEL和Feulgen′sDNA染色法观察伤后DNA片段化和DNA含量变化,可以应用于脑损伤时间推断的研究,探索推断损伤时间的新方法。
Objective To observe the changes of DNA fragmentation and its quantity along with the time of injury in nerve cells after brain contusion. Methods The model of brain contusion caused by free drop hammer was established. TUNEL and Feulgen＇s DNA staining conjoined with image analysis technique were used for exploration. Results With the gradually rising of DNA fragmentation,DNA quantity was declining in the brain tissue after contusion. Conclusion TUNEL and Feulgen＇s DNA staining conjoined with image analysis technique could be utilized in the timing of brain injury and provide a new approach for this issue.
出处
《法医学杂志》
CAS
CSCD
2006年第3期168-170,F0002,共4页
Journal of Forensic Medicine
基金
博士点基金资助项目(20030060000010)
