人体血液体外电击致血细胞穿孔的扫描电镜观察

Observation the Electroporation of Blood cells in Vitro by Scanning Electron Microscope

目的探讨血细胞体外电击时细胞膜的结构变化。方法对成人血液分别分组通过220V交流电电击5,10,20,30s和1min后,用扫描电镜观察血细胞膜的变化。结果电击后,即可以观察到红细胞与白细胞的细胞膜穿孔,其中红细胞穿孔可为一个或多个,形状多为圆形或卵圆形,白细胞穿孔则为多个,呈筛网状;穿孔细胞数随电击时间延长而增多;电击5s时为6%左右,以后逐渐增加,至电击1min时,穿孔细胞数可达40%左右。结论电流作用可以使血细胞膜发生穿孔,穿孔细胞数随通电时间延长而增多。

Objective To observe changes on cell membrane in blood cells after they were been electrified. Methods Blood were electrified for 5,10,20,30 s, lmin respectively, and Scanning electron microscope was used to detect the changes on their cell membranes. Results Pores were detected both on electrified erythrocytes and leukocytes with round or ellipse shapes. The erythrocytes often have one or more pores while the leukocytes often have more pores looked like cribble. The rates of perforated cells were increased with the prolonging time of electrification, 5 s with 6% and lmin increased to 40%. Conclusions Alternating current can cause the cell perforating, and the rates of perforated cell were increased with the prolonging time of electrification.