云南汉族人群11个Y-STR基因座遗传多态性调查及法医学应用

Polymorphisms of Eleven Y-chromosome STR loci and Forensic Application in Yunnan Han Population

目的调查11个Y-STR基因座及其单倍型在云南汉族人群中的遗传多态性分布,探讨其法医学应用价值,为法医学应用提供基础数据。方法应用Powerplex!Y系统对云南汉族201名无关男性个体进行11个Y-STR基因座的复合扩增,用ABI310型基因分析仪对扩增产物进行检测,统计其群体遗传学参数。结果Powerplex!Y系统前10个Y-STR基因座分别检出3、5、6、8、5、4、5、8、4、7个等位基因,DYS385a/b基因座检出56种单倍型;GD值最低为0.4273(DYS438),最高为0.9747(DYS385a/b);观察到11个Y-STR基因座共同构成的单倍型175种,其中有154种单倍型只出现1次,16种出现2次,5种出现3次,累计GD值为0.9984。结论11个Y-STR基因座具有较强的个体识别能力,可应用于云南地区汉族人群的个体识别与亲权鉴定。

Objective To abtain the genetic polymorphisms data of eleven Y-chromosome STR loci in Yunnan Han population, and to evaluate its forensic application values, which will provide the base data for forensic application. Methods DYS391,DYS389 Ⅰ ,DYS439,DYS389 Ⅱ ,DYS438,DYS437,DYS19, DYS392,DYS393,DYS390 and DYS385a/b loci were amplified by using Powerplex Y PCR amplification Kit and were analyzed by 310 genetic analyzer. 201 unrelated male individuals of Hart population in Yunnan were investigated to determine the distributions of allele frequencies and haplotypes. Results 3, 5,6,8,5,4,5,8,4,7 alleles were found across the Y-STR loci except DYS38556 haplotypes were found across DYS385a/b locus. The minimum GD value was 0.4273 （DYS438）,and the maximum was 0.9747 （DYS385a/b）. 175 haplotypes were found, of which 154 were unique, 16 were found in 2 individuals,5 were found in 3 individuals, and the observed haplotypes diversity value was 0.9954. Conclusion The 11 Y-STR loci are highly polymorphic and are suitable for forensic personal identification and paternity testing in Yunnan Han population .