食管癌组织染色体位点特异性的杂合性丢失和微卫星DNA序列不稳定

CHROMOSOME LOCI SPECIFIC LOSS OF HETEROZYGOSITY AND MICROSATELLITE INSTABILITY IN ESOPHAGEAL CANCER TISSUES

为了寻找在食管癌中发生缺失的染色体位点，根据以往细胞遗传学的工作基础，选取２４个分别位于１、２、３、５、７、９、１１、１７号染色体的食管癌染色体畸变＂热点＂及已知基因的位点，以微卫星序列－ＰＣＲ法检测这些位点处的杂合性丢失和微卫星序列不稳定。对３６对来自北京和山西阳泉的散发食管癌标本和相应正常组织的检测结果显示：在对应于９ｐ２２－２３、３ｐ１４．２、２ｐ２２、３ｐ２４－２６、１１ｑ１３．１、３ｐ２３、３ｐ２１．３、７ｑ３５、３ｑ２１－２２等染色体位点处存在较高频率（＞２０％）的杂合性丢失。同时，发现了染色体位点特异的微卫星ＤＮＡ不稳定。杂合性丢失和微卫星ＤＮＡ序列不稳定可能与食管癌的发生、发展过程有关。

To detect the loss of heterozygosity and microsatellite DNA instability in esophageal cancer tissues collected from northern China,we used PCR-silver stain method by 24 polymorphic microsatellite DNA markers on chromosomes 1,2,3,5,7,9,11 and 17.In 36 sporadic esophageal cancer samples and their paired normal tissue,LOH was detected on loci of D9S156(36.1%,9p22-23),D3S1480(30.6%,3p14.2),D2S131(29.4%,2p22),D3S587(26.5%,3p24-26),D11S480(25.7%,11q13.1),D3S647(25.7%,3p23),D3S966(23.5%,3p21.3),TCRB(22.9%,7q35) and D9S176(22.2%,9q22).Chromosome loci specific mirosatellite instability was found in all of 24 studied loci, especially on D9S156(30.6%),D3S966(29.4%),D3S647(28.6%),D3S1238(25.0%), D7S486(23.5%),and GSN(20.0%).It is suggested that loss of heterozygosity and microsatellite instability may play a role in the pathogenesis of esophageal cancer.