外源性p53基因对人胃粘膜上皮细胞系GES－1的生物学效应

BIOLOGICAL EFFECTS OF EXOGENIC p53 GENES ON HUMAN GASTRIC EPITHELIAL CELL LINE-CES-1

为了解ｐ５３基因在细胞癌变过程中的作用，分别将野生型和突变型的ｐ５３基因导入体外培养的人胃粘膜上皮细胞系ＧＥＳ－１中，进一步比较，分析转染ｐ５３基因对ＧＥＳ－１细胞的生物学效应发现：（１）外源ｐ５３基因改变了ＧＥＳ－１细胞的形态──转染野生型ｐ５３基因使得ＧＥＳ－１出现一些细胞分化的迹象，在其胞浆有明显的空泡，空泡周围有微管的环绕；（２）ｐ５３基因的转染改变了ＧＥＳ－１生长状态──野生型的ｐ５３基因转染的细胞的克隆形成率低于突变型ｐ５３基因以及空载体ｎｅｏ转染的细胞。实验还发现突变型ｐ５３基因改变了细胞生长习性，使细胞有选择生长优势，对细胞恶性度有促进作用。

To explore the role of p53 on the progress of cell carcinogenesis,the wild type or mutant-type p53 gene has been transfected into human gastric epithelial cell line-GES-1.Further investigation revealed that wt-p53 changed the morphogical features of cells-some cytoplasmic vaculoes as the sign of cell differentiation were found in the transfected cells and tbe vacuoles were surrounded by microtubulin in wt-p53 transfected cell, and that transfection of p53 gene altered the cell growth behavior-the colony formation rate of wt-p53 transfected cells were less than that of wt-p53 and neo vector transfectants.In addition,mt-p53 transfected cells got the anchorage independent growth ability.Tha results show that wt-p53 confers a selective growth advantage on transfected cells and hence leads to malignancy progression.