不同温度、pH和无机离子对黑曲霉A66菌株产柚苷酶活力影响的初步研究

The Effect of Temperature,pH and Ions on Activity of Naringinase Produced by Aspergillus niger A66

对黑曲霉产柚苷酶的最适温度和pH进行了研究，发现最适反应温度在60℃，最适反应pH在5．0左右。同时研究了不同的无机离子在不同浓度下对柚苷酶活性稳定性的影响，发现I^-，Zn^2＋能促进柚苷酶活性的提高，Cu^2＋在（0—0．004）mg／mL的范围内也能激活柚苷酶的活性。

The optimum temperature and pH for naringinase were studied. The results show that the optimum temperature is 60 ℃ and the pH is 5.0. The effect of ions was also studied and it was observed that I^-, Zn^2＋ can improve the activity of naringinase, and in the concentration range of （0 -0. 004）mg/mL, Cu^2＋ can also improve the activity.