高效液相色谱法测定金钱草颗粒中槲皮素和山奈素的含量

Determination of Quercetin and Kaempferol in Herba Lysimachiae Granules by HPLC

目的建立用高效液相色谱(HPLC)法测定金钱草颗粒中槲皮素和山奈素含量的方法。方法采用Dalian E lite Hy-persil ODS2 5μm(200 mm×4.6 mm)柱,以甲醇-0.4%磷酸溶液(45∶55)为流动相;流速:1.0 m l.m in-1;检测波长为360nm。柱温:30℃。结果该法线性关系良好,槲皮素平均回收率为101.2%,RSD为1.14%;山奈素平均回收率为100.1%,RSD为1.33%。结论该方法简便、准确、重现性好,可用于该制剂的质量控制和评价。

Objective To establish an HPLC method for the determination of quereetin and kaempferol in Herba Lysimachiae Granules. Methods Dalian Elite Hypersil ODS2 5μm（200 mm ×4.6 mm） column was used. The mobile phase was methanol - 0.4% phosphoric acid solution （45： 55）. The flow rate was 1.0 ml · min^-1 ;The detection wavelength was 360 nm;The column temperature was 30℃. Results The linear relation was good . The average recovery of quercetin was 101.2% with RSD = 1.14% ;The average recovery of kaemferol was 100.1% with RSDwas 1.33%. Conclusion The method is simple,accurate and with a good reproducibility and can be used for the quality control of the granules.