摘要
目的 用高效液相色谱法测定骨宝昕肾片中染料木素的含量。方法 采用Zoobax C18(4.6mm×250mm)色谱柱,乙腈.水(V:V=30:70)为流动相,检测波长270nm,柱温40℃,流速1.0ml/min。结果 线性范围0.001 752~0.019 272μg.线性关系良好,回归方程为Y=4866020.692X-20.276,相关系数r=0.9999,平均回收率为98.89%,RSD为0.91%。结论 该法适合测定骨宝昕肾片中染料木素的含量。
Objective To determine the content of the genistein in the Gubaoxinshen Tablets by HPLC. Methods The Zoobax C18 Column(4.6 mm ×250 mm)was used,the methyl cyanide -water(V: V =30: 70) mixture as the mobile phase,with the detection at 270 nm ,the flow rate was 1.0 ml/min and column temperature was 40℃. Results The linear calibration curves were obtained in the concentration ranges of 0.001 752 - 0.019 272 μg. The regression equation was Y =4 866 020.692 X- 20.276, r = 0. 999 9. The average recovery was 98.89% ,and RSD was 0.91%. Conduslon This method was convenient,accurate and specified.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第6期982-983,共2页
Lishizhen Medicine and Materia Medica Research