荧光分析法测定黄花参肝舒茶中大黄素的含量

Determination of Emodin in Content Huanghuashen Ganshu Tea by Spectrofluorimetry

目的荧光分析法在黄花参肝舒茶中测定大黄素含量方法探讨。方法采用荧光分析法,吸附剂为硅胶G,展开剂为石油醚-醋酸乙酯-甲酸(15∶5∶0.5),激发光波长214 nm,荧光光谱波长569 nm进行测定。结果大黄素线性回归方程为:Y=1.532 3X+552.14,r=0.998 6;平均回收率为98.88%,RSD值为2.16%(n=5)。结论该方法灵敏度高、操作简便、重现性好、结果准确,可用于黄花参肝舒茶制剂的质量控制。

Objective To develop protocol for quality control of Huanghuashen Ganshu tea by spectrofluofimetry. Methods Spectrofluofimetry was carried out on silica G with petroleum aether-acetate-formic acid（ 15： 5：0.5 ）as developing agents. The Excitation wavelength was 214nm, fluorescence spectra were measured at 569nm. Results Emodin regression equation： Y = 1. 532 3X ＋ 552 . 14 ,r = 0. 998 6;Average recovery was 98. 88% and RSD = 2.16%. Conclusion The method was simple and feasible and easy for operation. It can be used as an evidences for quality control of Huanghuashen tea.