适宜大叶黄杨茎段不同组织培养阶段的培养基研究

A Study on Stem Tissue Culture Medium Suitable for Different Culture Stage of Buxus megistophylla

以MS为基本培养基,附加不同种类、浓度的生长调节剂,通过试验寻求适宜于大叶黄杨茎段各组织培养阶段的培养基。研究结果表明:在诱导培养阶段,较低浓度的细胞分裂素能促进大叶黄杨茎段侧芽的萌发,其适宜的诱导培养基为MS+BA0.5 mg/L;继代增殖阶段,以MS+BA1.0 mg/L+NAA0.1 mg/L+GA32.0 mg/L为培养基效果较好;生根阶段,最适宜的培养基为1/2 MS+NAA0.5 mg/L。

Taking MS as the basic medium, adding different plant growth regulators of different concentrations, the tissue culture medium of Buxus megistophylla at different culture stage was studied. The results of study indicated that at the inducing stage, low concentrated cytokinin was benefit for the formation of lateral bud, and the suitable medium was MS ＋ BA 0. 5 mg/L, the suitable medium for subculture was MS ＋ BA 1.0 mg/L ＋ NAA 0. 1 mg/L ＋ GA32. 0 mg/L, and the recommended medium for rooting stage was 1/2 MS ＋ NAA 0. 5 mg/L.