摘要
建立了测定兔血清中硫酸多糖药物聚甘古酯的高效液相-柱后衍生荧光色谱方法。以盐酸胍为衍生试剂,采用离子排阻柱Aminex HPX-87H(300mm×7.8mm),0.005mol·L^-1H2SO4为流动相,流速为0.5mL·min^-1,荧光检测,外标峰高法定量。方法线性范围为10~100μg·mL^-11。高、中、低3种浓度的日内及日间精密度的RSD分别为4.28%,4.05%,5.97%。6.78%,5.32%,7.68%。高、中、低3种浓度的相对及绝对回收率平均值分别为92.32%,94.76%,94.44%,72.07%。73.42%,70.77%。本方法可准确、快速测定兔血清中聚甘古酯的浓度,为其药物动力学研究提供了1个灵敏、可靠的分析方法。
To develop a method for the determination of poly-mannugluronic acid in rabbit serum by HPLC post-column fluorescent derivatization, an Aminex HPX-87H (300mm × 7.8mm) column was used, with guanidine hydrochloride as derivative reagent. The mobile phase was 0.005mol·L^-1 H2SO4 at a flow rate of 0.5mL·min^-1. The content of Poly-mannugluronic was quantitated by peak height of the external standard. The linearity was shown to be the range of 10-100μg·mL^-1. The inter-day and intra-day RSDs of three concentrations were less than 8 %. The absolute recovery was within 70.77 %-73.42 %. This method is rapid, accurate and applicable to the study of pharmacokinetics and bioavailability of poly-mannugluronic acid.
出处
《中国海洋大学学报(自然科学版)》
CAS
CSCD
北大核心
2006年第B05期79-82,共4页
Periodical of Ocean University of China
基金
国家高技术研究发展计划项目(2002AA624020)资助