摘要
建立了测定兔血清中硫酸多糖药物聚甘古酯的高效液相-柱后衍生荧光色谱方法。以盐酸胍为衍生试剂，采用离子排阻柱Aminex HPX-87H（300mm×7．8mm），0．005mol·L^-1H2SO4为流动相，流速为0．5mL·min^-1，荧光检测，外标峰高法定量。方法线性范围为10～100μg·mL^-11。高、中、低3种浓度的日内及日间精密度的RSD分别为4．28％，4．05％，5．97％。6．78％，5．32％，7．68％。高、中、低3种浓度的相对及绝对回收率平均值分别为92．32％，94．76％，94．44％，72．07％。73．42％，70．77％。本方法可准确、快速测定兔血清中聚甘古酯的浓度，为其药物动力学研究提供了1个灵敏、可靠的分析方法。
To develop a method for the determination of poly-mannugluronic acid in rabbit serum by HPLC post-column fluorescent derivatization, an Aminex HPX-87H （300mm × 7.8mm） column was used, with guanidine hydrochloride as derivative reagent. The mobile phase was 0.005mol·L^-1 H2SO4 at a flow rate of 0.5mL·min^-1. The content of Poly-mannugluronic was quantitated by peak height of the external standard. The linearity was shown to be the range of 10-100μg·mL^-1. The inter-day and intra-day RSDs of three concentrations were less than 8 %. The absolute recovery was within 70.77 %-73.42 %. This method is rapid, accurate and applicable to the study of pharmacokinetics and bioavailability of poly-mannugluronic acid.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第B05期79-82,共4页
Periodical of Ocean University of China
基金
国家高技术研究发展计划项目(2002AA624020)资助
