兔眼虹膜色素上皮细胞的培养与鉴定

Cultivation of rabbit iris pigment epithelia and identification of its characteristics

目的:体外培养和观察兔眼虹膜色素上皮细胞(IPEC),为临床和实验研究IPE细胞提供基础。方法:采用酶消化加酶辅助机械分离法分离IPE细胞,观察培养细胞的生长情况,并采用免疫荧光法对其进行鉴定。结果:原代培养的IPE细胞多数呈圆形或六边形,细胞内充满黑色颗粒。传代培养的IPE细胞呈梭形或纤维细胞样生长,细胞中的色素颗粒也逐渐减少。免疫荧光法对培养细胞进行cytokeratin染色显示IPE细胞成阳性反应,阳性率达100%。结论:采用酶消化加酶辅助机械分离法可成功地分离培养出IPE细胞。

AIM： To isolate and culture iris pigment epithelia （IPE） cells of rabbits in vitro for the base of clinical and experimental application. METHODS： IPE cells of rabbits were isolated by enzymatic digestion method and enzyme-assisted microdissection method. The cultivation of IPE cells was observed and the cultured IPE cells were identified by immunocytochemical method. RESULTS： Separated IPE cells showed as round and polygonal with were a great number of round, pigmented granules in them. When the cells were passaged into subcultures, the pigment granules were gradually lighter and the spindie-shaped or fiber-like. IPE cells stained positive for cytokeratin. CONCLUSION： Enzymatic digestion method combined with enzyme-assisted microdissection method is reliable and successful for the isolation of IPE.