肾移植受者血清IL-10测定的临床意义

Evaluation of IL-10 in Renal Allograft Recipients

目的观察肾移植受者术后肾功能稳定、发生排异反应、感染、慢性移植肾肾病(CAN)时血清白细胞介素-10(IL-10)的水平;检测肾功能稳定者肾移植术后不同时间、环孢菌素A(CsA)剂量、排异次数时血清IL-10水平,以探讨IL-10在肾移植受者门诊随访中的意义。方法采用双抗体夹心酶联免疫吸附法,随机对127例门诊肾移植术后随访者血清IL-10水平进行检测。以20例健康志愿者作为对照组。结果肾功能稳定组患者的血清IL-10水平明显高于术后排异组、CAN组和对照组,相比较均有显著统计学意义(P<0.05);肾功能稳定组中CsA用量每千克体质量2 mg组的血清IL-10水平明显高于4 mg组,相比有统计学意义(P<0.05);该组中移植术后无排异者的血清IL-10水平明显高于1次排异和多次排异者,相比均有统计学意义(P<0.05)。结论血清IL-10水平可部分反映移植患者的免疫状态,对肾移植受者门诊随访有一定的参考价值。

Objective To study the level of serum IL-10 in renal allgraft recipients with stable renal function,acute rejection, infection and chronic allgraft nephropathy （CAN） , and to study the level of serum IL-10 in stable renal function recipients with different postoperative time, different dosage of CsA, different rejective frequency in order to find out the value of IL-10 in the follow-up of out-patients. Methods IL-10 were detected randomly by ELISA technique in 127 renal allgraft recipients during the follow-up, and 20 healthy volunteers were enrolled as controls.Results Level of serum IL-10 in stable renal function group was significantly higher than that in rejection group,CAN group and control group（P 〈0.05）. In stable renal function recipients, serum IL-10 level was much higher in patients with 2 mg/kg CsA than that in patients with 4 mg/kg CsA （P 〈 0.05）, and serum IL-10 level was obviously higher in patients without rejection than that in patients with at least one rejection （ P 〈 0.05 ）. Conclusion The level of serum IL-10 can partially reflect the immune state of renal allgraft recipients, which have some reference value in the follow-up of out-patients.